Coding

Part:BBa_K4467012

Designed by: Eloise Paine   Group: iGEM22_UNSW_Australia   (2022-10-10)


SERK3B-mVenus fusion protein

Part:BBa_K4467012 is a fusion protein featuring a new part by the 2022 UNSW iGEM team, SERK3B (Part:BBa_K4467006), and existing part, mCerulean (Part:BBa_K323023). For more information detailing how this fusion protein was modelled and designed, see our team wiki page. Link: https://2022.igem.wiki/unsw-australia/model Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2080
    Illegal PstI site found at 1069
    Illegal PstI site found at 2377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2080
    Illegal NheI site found at 1915
    Illegal PstI site found at 1069
    Illegal PstI site found at 2377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2080
    Illegal BglII site found at 1161
    Illegal BamHI site found at 1596
    Illegal XhoI site found at 852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2080
    Illegal PstI site found at 1069
    Illegal PstI site found at 2377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2080
    Illegal PstI site found at 1069
    Illegal PstI site found at 2377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1999
    Illegal BsaI.rc site found at 2356
    Illegal SapI site found at 87
    Illegal SapI.rc site found at 82


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Parameters
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